ZFIN Image: Nagelberg et al., 2015, Fig. S3

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Figure Caption

Fig. S3

Related to Figure 5. Efficient specification of pPAA progenitors requires the combined activity of Tcf21 and Nkx2.5.

(A-D) 36 hpf tcf21:mCherry-NTR embryos of indicated genotypes stained for tie1 mRNA (red) and mCherry (cyan) expression. The mCherry and tie1 fluorescent signals are shown separately in A′-D′ and A′′-D′′, respectively. Numbers in A-D indicate the pharyngeal arches 2-6. Note that the tcf21+ cells in the first two pharyngeal arches are dying in embryos without Tcf21 function. Anterior is to the left and dorsal is up. The scale bar in D corresponds to 100 µm. Wild-type embryos, n = 19 (includes tcf21-/+ and or nkx2.5-/+ embryos), tcf21-/- embryos n = 8 (includes nkx2.5-/+ embryos); nkx2.5-/- embryos (includes tcf21-/+ embryos), n = 5; tcf21-/-; nkx2.5-/- embryos, n = 3. tie1 signal is shown only when colocalizing with mCherry, as detected using a custom-written imageJ macro (Data S3).

Acknowledgments

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