Fig. 2

Fig. 2

A subset of Wnt/PCP molecules influence craniofacial cartilage development. (A–F) Not all identified core Wnt/PCP zebrafish mutants have a craniofacial chondrocyte stacking defect. Confocal images of whole-mount Wheat Germ Agglutanin (WGA) staining (gray) of Wnt/PCP mutant embryo heads at 4 dpf. Scale bar = 50 µm. (G–L) Confocal images of a single focal plan of WGA staining (gray) of Wnt/PCP mutant craniofacial cartilage elements at 4 dpf. Nuclei were labeled with DAPI (blue). Scale bar = 20 µm. M. Graph of length to width ratio. The y-axis is all 40 chondrocyte measurements from 4 different ceratohyals for wild type and mutant genotypes. The x-axis is the length to width ratio with a graphical representation of select ratios. The mean LWR and their respective standard deviation is marked by red cross-hairs and stated in Supplemental Table 1. To determine if data could be compared pairwise the D′Agostino and Pearson omnibus normality test was used. Mann–Whitney U was determined and the statistical significances between the means is marked on graph and stated in Supplemental Table 1. Abbreviations: M = Meckel′s cartilage, CH = ceratohyal, CB = ceratobranchial, HM = hyomandibular, IH = interhyal joint, and PQ = palatoquadrate. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)