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Fig. S1
Validating specific inhibition of vcana-MO used in zebrafish embryos. The vcana-MO (12 ng; aMO) combined with its counterpart mRNA (50 pg) was injected into one-celled stage of zebrafish embryos. Uninjected embryos (A), embryos injected with eGFP mRNA alone (B), and embryos injected with eGFP mRNA plus vcana-MO (C), which served as the control group. The aMO-target-eGFP fusion protein was detected at 24 hpf in embryos injected with a aMO-target-eGFP mRNA (D). The aMO-target-eGFP fusion protein was nearly undetectable at 24 hpf in embryos injected with aMO-target-eGFP mRNA plus vcana-MO (E).
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