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Fig. S1 cup mutant KV volumes. Whole KV live-microscopy scans of 10–11 s.s. embryos from cup+/- ; foxj1a:GFP parents. The middle focal plan along the XY axis and the respective orthogonal views (along XZ and YZ axes) are shown for the most representative normal tail (cup+/-; cup+/+) (A), and curly-up tail (cup-/-) (B) embryos. KVvolume is indicated in µm3 and in picoL. Scale bars: 10 µm. (C) Estimated KV volumes (µm3) for normal tail (cup+/-; cup+/+) (n = 23) and curly-up tail (cup-/-) (n = 15) embryos. Average values and the respective s.d. are indicated.
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