ZFIN Image: Gokey et al., 2015, Fig. S10

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Figure Caption

Fig. S10 Atp6ap1b MO does not increase apoptosis. (A) Uninjected control embryos were treated with DNAse I to induce apoptosis and then used as positive control for annexin V labeling of apoptotic cells in Tg(sox17:GFP) embryos. (B,C) Annexin V labeling did not detect apoptotic DFCs in control MO (B) or atp6ap1b MO (C) injected embryos. (d-E) p53 MO was co-injected with control MO or atp6ap1b MO to determine if blocking apoptosis would rescue atp6ap1b MO phenotypes. Embryos co-injected with atp6ap1 MO-1+p53 MO showed defects in KV size (D) and cilia number (E) that were similar to those observed in embryos injected with atp6ap1 MO-1 alone (see Fig. 2).

Acknowledgments

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Reprinted from Developmental Biology, 407(1), Gokey, J.J., Dasgupta, A., Amack, J.D., The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish, 115-30, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.