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Fig. 5
VEGFR deficiency-associated vascular phenotypes and functional rescue by Vegfba or Vegfaa mRNAs. (A) Bright-field and confocal micrographs of 24- and 72-hpf fli1a:EGFP zebrafish embryos injected with 0.6 pmol of Scrambled, Vegfr1, Vegfr2, or Nrp1 morpholinos. Black arrow indicates zebrafish brain with dead cells. White arrows indicate 24-hpf MCVs and the 72-hpf brain vasculatures. Yellow arrows indicate ISVs. Yellow X symbols indicate absence of ISVs. (Scale bars in overviews, 500 µm; in brain and periphery, 50 µm.) (B) Quantifications of 24-hpf MCV length, ISV length, 72-hpf brain vascular density, and percentage of defective ISVs after treatment with 0.6 pmol of Scrambled (Ctrl), Vegfr1 (R1), Vegfr2 (R2), or Nrp1 morpholinos (average of n = 14 embryos/group). NS, nonsignificant. **P < 0.01, ***P < 0.001. (C) Confocal images of 24- and 72-hpf fli1a:EGFP embryos injected with 0.6 pmol of Scrambled or Vegfba morpholinos alone or in combinations with 100 ng Vegfba or Vegfaa mRNAs. White arrows indicate 24- MCVs and 72-hpf brain vasculatures. White X indicates absence of the MCV. (Scale bars, 50 µm.) (D) Quantification of 24-hpf MCV length and 72-hpf brain vascular density of fli1a:EGFP embryos injected with 0.6 pmol of Scrambled (Ctrl) or Vegfba (Ba) morpholinos alone or in combinations with 100 ng Vegfba or Vegfaa (Aa) mRNAs (average of n = 16 embryos/group). ***P < 0.001.