|
Fig. 1
Ocular abnormalities in cloche mutant embryos. A?F: Confocal images of kdrl:EGFP wild-type (A,C,E) and clo-/- (B,D,F) blood vessels (green). Hyaloid artery (ha) has invaded the eye and superficial vessels (sv) begin to form at 29 hpf in wild type (A) but not clo-/- (B). C,D: Hyaloid capillaries (hc), and superficial vasculature (sv) have developed at 54 hpf in wild-type (C) but not clo-/- (D). E,F: Branchial arch vessels (bav) are present at 54 hpf in wild-type (E), and are strongly reduced in clo-/- (F). G,H: Confocal images of Fast Red staining of endogenous alkaline phosphatase; focal plane of hyaloid capillaries, which are present in wild-type (G) but not clo-/- (H). I-L: Live, wild-type (I, K) and clo-/- (J,L) embryos imaged at 36 hpf (I,J) and 48 hpf (K,L). M?P: Eye diameters and lens diameters of wild-type and clo-/- embryos at 30 (M), 36 (N), 48 (O) and 72 (P) hpf; ***P < 0.001. Scale bars = 50 µm in A (applies to A,B); in C (applies to (C?F); in G (applies to G,H).