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Fig. S5
Knockdown of irf4a turns irf4a-expressing lymphoid cells to lyz-expressing myeloid cells without affecting erythroid lineage.
(A) The expression of erythroid cells markers, gata1 and βe1-globin in the control and irf4a morphants at 4 dpf. The red arrows indicate cells expressing gata1 or βe1-globin. (B) The expression of coro1a in the control and irf4a morphants at 3 dpf examined by WISH. Black arrowhead indicates the thymus and red arrow indicates coro1a-expressing cells in the CHT. (C) qPCR analysis of coro1a expression in the control and irf4a morphants at 3 dpf. ns, not significant. (D) Confocal images of irf4a-expressing cells in the CHT region of the irf4a:GFP transgenic embryos at 60 hpf (scale bar, 80 µm). The magnified view of irf4a-expressing cell is shown in the right panels (scale bar, 10 µm). (E) Still images of a time-lapse imaging of double transgenic (irf4a:GFP/ lyz:DsRed) wild-type and irf4a morpholino injected embryos. The time-lapse series begins at 58 hpf. White or red arrows indicate the same cell in the control or irf4a morphants respectively (scale bar, 40 µm). All data are means ± SD.
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Reprinted from Developmental Cell, 34(6), Wang, S., He, Q., Ma, D., Xue, Y., Liu, F., Irf4 Regulates the Choice between T Lymphoid-Primed Progenitor and Myeloid Lineage Fates during Embryogenesis, 621-31, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell