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Fig. S6
The increase of coro1a+ cells in the CHT of irf4a morphants is pu.1 dependent.
(A) The expression of coro1a in the CHT of the control, irf4a morphants and irf4a + pu.1 morphants at 3 dpf examined by WISH. Red arrows indicate coro1a-expressing cells in the CHT. (B) The expression of pu.1 in the CHT region of the control and embryos injected with HSP-Irf4a CDS examined by WISH. Red arrows indicate pu.1-expressing cells in the CHT. (C) Reporter assay showed the regulation of pu.1 promoter by Irf4a CDS. Pu.1 pro indicates the promoter of pu.1 with Irf4 binding site used in ChIP assay (Figure 6D). * p<0.05, ** p<0.01,*** p<0.001. All data are means ± SD.
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Reprinted from Developmental Cell, 34(6), Wang, S., He, Q., Ma, D., Xue, Y., Liu, F., Irf4 Regulates the Choice between T Lymphoid-Primed Progenitor and Myeloid Lineage Fates during Embryogenesis, 621-31, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell