ZFIN Image: Cort�s-Campos et al., 2015, Fig. 2

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Figure Caption

Fig. 2

Neurospheres can be generated from the hypothalamus of adult zebrafish. (A) Neural progenitors were disaggregated (black cells), plated, and maintained in proliferation media for 7day (A1 to A3 neurospheres, 7 to 0days). Cells were then seeded (A4 to A6, upper panel) on coated chamber wells and changed to differentiation media for 7days (0 to +7days). Alternatively, neurospheres (A3) were disaggregated and seeded (A4 to A6, bottom panel) on coated chamber wells and changed to differentiation media for 7days with or without hormonal treatment (0 to +7days). (B-D) 7days in vitro bright field images of neurospheres (A3). Scale bar 30�m.

Acknowledgments

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