ZFIN Image: Cortés-Campos et al., 2015, Fig. 2

Image

Figure Caption

Fig. 2

Neurospheres can be generated from the hypothalamus of adult zebrafish. (A) Neural progenitors were disaggregated (black cells), plated, and maintained in proliferation media for 7day (A1 to A3 neurospheres, 7 to 0days). Cells were then seeded (A4 to A6, upper panel) on coated chamber wells and changed to differentiation media for 7days (0 to +7days). Alternatively, neurospheres (A3) were disaggregated and seeded (A4 to A6, bottom panel) on coated chamber wells and changed to differentiation media for 7days with or without hormonal treatment (0 to +7days). (B-D) 7days in vitro bright field images of neurospheres (A3). Scale bar 30µm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Open