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Fig. S3
Endocrine cell phenotype following the inhibition of polyamine biosynthesis by DFMO treatment.
Representative images at 72 hps of glucagon-expressing cells in (a) control and (b) DFMOtreated Tg(ptf1a:gfp) embryos; insulin-expressing cells in (c) control and (d) DFMO-treated Tg(ptf1a:gfp) embryos; and insulin/glucagon co-expressing cells in (e) control and (f) DFMOtreated Tg(ptf1a:gfp) embryos. (g) Glucose measurement from control or DFMO-treated embryos at 72 hpf (n = 3 groups of 10 embryos/group) (p = 0.4821). (h) Western blot analysis for expression of DHS, eIF5AHyp, and Erk1/2 in control and DFMO-treated embryos at 72 hpf (n = 4 groups of 30 embryos/group); Erk1/2 was used as a loading control, as expression of this protein is not altered with DFMO treatment. Quantification showed no change in (i) DHS expression (p = 0.2370) but a significant decrease in (j) eIF5AHyp expression (p = 0.0039). gluc, glucagon; ins, insulin. * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant.
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