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Fig. S2
Titration of DFMO in zebrafish.
(a) Percentage of viable embryos in control (n = 180), 1% w/v DFMO-treated (n = 33), 2% w/v DFMO-treated (n = 45), 3% w/v DFMO-treated (n = 28), 5% w/v DFMO-treated (n = 24), and 10% w/v DFMO-treated (n = 15) embryos at 72 hpf. (b) Percentage of control (n = 32), 1% w/v DFMO-treated (n = 33), 2% w/v DFMO-treated (n = 40) embryos at 72 hpf with altered body axis due to DFMO treatment. (c) Representative images of control and DFMO-treated Tg(ptf1a:gfp) embryos displaying the phenotype of altered (truncated) body axis. (d) The ptf1a: GFP-expressing retina (r) and hindbrain (hb) were unaltered in DFMO-treated Tg(ptf1a:gfp) embryos compared with controls, at 48 hpf and 72 hpf.
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