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Fig. 3
Localization of aqp1a.1
Triple-labeling of aqp1a.1 mRNA (with in situ hybridization), H+-ATPase (HA), and Na+/K+-ATPase (NKA) (with immunofluorescence) in 3-dpf embryos. The aqp1a.1 antisense probe labeled ionocytes in the yolk-sac skin of embryos (A, D). Those signals were not found in the negative control with an aqp1a.1 sense probe (B). aqp1a.1 signals (arrows) were also found in developing gills (C). The yolk-sac aqp1a.1 signals (D) were co-localized with HA (red arrows in E) and NKA (green arrows in E). A merged image of (D) and (E) is shown in (F).
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