ZFIN Image: Li et al., 2015, Fig. 6

Image

Figure Caption

Fig. 6

Tet2/3 Regulate Expression of the gata2b/scl/runx1 Transcriptional Network in the Hemogenic Endothelium

(A and B) WISH for scl at 31 hpf.

(C and D) WISH for runx1 at 24 hpf.

(E and F) WISH for gata2b at 24 hpf.

(G and H) WISH for gata2a at 24 hpf.

(I and J) WISH for runx1 in the DA of mock-injected and sclβ mRNA-injected tet2/3^DM embryos at 36 hpf.

(K and L) WISH for runx1 in the DA of mock-injected and gata2b mRNA-injected sibling embryos at 36 hpf.

(M) Percent of tet2/3^DM embryos with high runx1 expression following mock injection or injection with mRNA encoding Sclβ (50 pg) or Gata2b (200 pg). Numerical data are presented as the mean ± SEM.

Numbers in the lower right corner of images indicate the fraction of larvae with WISH labeling similar to the representative image. All scale bars indicate 50 µM. See also Figure S4.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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