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Fig. S7
Exposure to BN and BC194 results in vascular defects and mis-patterning.
(a) Representative CAM images showing rapid changes in vessel directionality, indicated by black arrows, before and after 72 hour treatment with PBS control, 1 µM BN, or 1 µM BC194. (b) Whole body images of 24 hpe zebrafish embryos treated with various concentrations of BN, BC194, and BC220 for 24 h. Extra DMSO was added to all samples, including 0 µM, as required to match the highest concentration used for each compound (0.025% for BN and BC194 and 0.1% in BC220). (c, d) Histograms showing the effects of BN, BC194, and BC220 exposure on zebrafish body length (c) and heart rate (d). Dechorionated embryos were incubated for 24 hours in egg water containing the indicated compounds. Body length measurements were obtained by analysis of bright-field images using Spot v 5.1 software; mean ± SEM, n=11, *p<0.05 relative to 0 nM (one-way ANOVA, Tukey Test). Heart rates were determined using bright-field microscopy to count heart beats over 15 second intervals; mean ± SEM, n=16, *p<0.05 relative to 0 nM (one-way ANOVA, Tukey Test).
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