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Fig. S10
Depletion of TARS elicits a similar vascular phenotype to BN and BC194 inhibition.
(a-b) RT-PCR (a) and RT-qPCR (b) of trizol/chloroform extracted mRNA from uninjected controls (UIC) and TARS morphants (MO) validate the reduction of TARS levels by morpholino injection. (a) Whole fish mRNA (48 hpe) was amplified by RT-PCR with gene specific primers. The TARS (338 bp) and EF1a control amplicons were run on 1.5% agarose to show differences in relative intensities between uninjected controls (UIC) and morphant fish. (b) cDNA was generated from isolated mRNA using oligo-dT primers and the tars expression relative to EF1α was determined by the ΔΔCT method; mean ± SEM, n=2, *p<0.05 relative to UIC (unpaired t-test). (c) Full size gel for image shown in a. (d) Whole body images of UIC and TARS morphant fish at 24 and 48 hpe.
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