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Fig. 1
The sphk2 gene is maternally expressed. Shown are representative wildtype embryos analyzed for sphk2 transcripts by in situ hybridization at the stages indicated: (A) one-cell stage, (B) 1,000-cell stage (3 hpf), (C) sphere stage (blastula, 4 hpf), and (D) shield stage (gastrulation, 6 hpf). (E) At 24 hpf, sphk2 is expressed ubiquitously. In contrast (F–J), sphk1 transcripts are not detected above background by in situ hybridization until somitogenesis.
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