|
Fig. 5
Injections of WT zebrafish plasma rescue the hyperlipidemia phenotype in apoc2 mutants. (A) apoc2 WT or mutant larvae at 5dpf were injected with 5nl of undiluted plasma from either WT or mutant (mut) adult male zebrafish, both supplemented with red fluorescent dextran. At 1 day after injection, at 6dpf, larvae were stained with BODIPY (green fluorescence) to assess the neutral lipid content of plasma. Red dextran fluorescence indicates successful injection. Scale bar: 100µm. (B) Quantification of results shown in A. Results are mean±s.e.m.; n=3 in each group; ***P<0.001; ns, not significant (Student′s t-test). (C,D) TG and TC levels in homogenates of 6dpf WT and apoc2 mutant larvae, normalized to total protein content. Results are mean±s.e.m.; five embryos pooled per sample, n=3 in each group; *P<0.05; **P<0.01 (Student′s t-test).