ZFIN Image: Riemer et al., 2015, Fig. 3

Image

Figure Caption

Fig. 3

Buc-GFP transgenic zebrafish. (A) Scheme showing the genomic Buc locus used to generate the Buc-GFP line. Thin lines represent non-coding regions. Boxes represent exons (white: untranslated RNA; red: coding RNA). The green box marks the insertion point of the GFP-protein in front of the STOP-codon. (B–D) Live images of Buc-GFP (green) in oocytes from transgenic mothers at early stage IB (B), late IB (C), and II (D). The nuclei of follicle cells were counterstained with the live-cell fluorescent dye Hoechst 33342 (blue). (E–G) Confocal images comparing endogenous Buc localization with Buc-GFP. (E) Wild-type stage IB oocyte labeled with Buc antibody (white) compared to transgenic Buc-GFP oocyte labeled with GFP antibody (F; green) and Buc (G; white). Dashed yellow line indicates oocyte outline (E–G). Lateral view, animal to the top. Scale bar: 10 µm (E–G) 25 µm (B–D).

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Gene expression patterns : GEP, 18(1-2), Riemer, S., Bontems, F., Krishnakumar, P., Gömann, J., Dosch, R., A functional Bucky ball-GFP transgene visualizes germ plasm in living zebrafish, 44-52, Copyright (2015) with permission from Elsevier. Full text @ Gene Expr. Patterns