ZFIN Image: Yang et al., 2015, Fig. S5

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Figure Caption

Fig. S5

plk2a and plk2b expression in cloche mutant embryos. (A, C) Whole mount RNA in situ hybridization in wild-type sibling cloche^m39 control embryos shows that (A) plk2a is expressed broadly in the brain and nervous system (yellow arrowheads) and weakly in the vasculature (white arrowheads) (n=21/21), whereas (C) plk2b is expressed primarily in the vasculature (n=19/19) at 36 hpf. (B, D) However, in cloche^m39 mutants, which lack endothelial cells, plk2b (n=12/12) and plk2a (n=10/11) expression is reduced in the vasculature, but plk2a appears to be still expressed in the brain and nervous system at 36 hpf. A′–D′ are enlarged images of the boxed area of A–D. (E) Quantitative PCR shows that plk2b (p=0.003) but not plk2a (p=0.4096) expression is redcued in the cloche^m39 when compared to the wild-type siblings. Mean±s.e.m. **p<0.01 by Student′s t-test.

Acknowledgments

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Reprinted from Developmental Biology, 404(2), Yang, H., Fang, L., Zhan, R., Hegarty, J.M., Ren, J., Hsiai, T.K., Gleeson, J.G., Miller, Y.I., Trejo, J., Chi, N.C., Polo-like kinase 2 regulates angiogenic sprouting and blood vessel development, 49-60, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.