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Fig. 6
Pou5f1 modification by phosphorylation. Western blot analysis of 70% epiboly stage embryo extracts treated at 37°C with phosphatase inhibitor (PI) or alkaline phosphatase (AP) for defined periods (indicated at bottom). Pou5f1-positive protein bands were detected around 60 kDa in wild-type (WT) but reduced to a band of approximately 57 kDa molecular mass upon AP treatment. Anti-α-tubulin was used as loading control (red, 55 kDa).
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