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Fig. 3
Pou5f1 in dorsal ectoderm cell nuclei in wild-type (WT) embryos at one-somite stage. A–C: Confocal images of anti-Pou5f1 immunofluorescence (green) WT whole-mount embryos at one-somite stage in which all cell nuclei were stained with TOTO-3 (red). A′–C′: Higher magnification of rectangle area in A is shown. Arrows indicate region from which on reduced Pou5f1 levels are detected in the caudal neural plate. Dorsal views, animal pole to the top.
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