Fig. S2

Fig. S2 Effect of neutrophil infiltration on expression of Laminin and collagen I in the liver. 8-dpf fabp10+ and kras+ larvae after exposure to FPR-A14 or PR-39 with or without doxycycline were cryo-sectioned and immune-stained for Laminin and Collagen I expression. (A, B) Immuno-staining for Laminin (A) and quantification (B). (C, D) Immuno-staining for Collagen 1a (C) and quantification (D). Control fabp10+ larvae with DsRed expression in hepatocytes were stained with Alexa Fluor 488-conjugated secondary antibody after the primary antibody incubation (top rows) while kras+ larvae with GFP expression in hepatocytes were stained with Alexa Fluor 568-conjugated secondary antibody (bottom rows). All sections were counter-stained with DAPI. Treatment groups (n=10 each group) and color probes are indicated at the top and on the right respectively. Quantification of percentage of Laminin- or collagen I-positive areas out of total liver area is presented in (C) and (D). Statistical significance in quantification: *p<0.05.