ZFIN Image: Gao et al., 2015, Fig. 5

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Figure Caption

Fig. 5 The apoptosis of HSPCs is p53 dependent in topbp1^cas003 mutants.

(A) Quantitative PCR results of c-myb, p53, p21, cyclin G1 and mdm2 in the CHT region of wild-type sibling and topbp1^cas003 mutant embryos at 3dpf. All these p53 dependent apoptosis-related genes are upregulated. Error bars represent SEM. *, p<0.05; ***, p<0.001. (B) Quantitative analysis of HSPCs phenotype, monitored by c-myb WISH analysis, showing inactivation of p53 can partially rescue c-myb expression in topbp1^cas003 mutants. The 3 kinds of standards of c-myb WISH results in the CHT at 4dpf are shown in the right bottom. The numbers of embryos are shown above the columns. (C-F′) c-myb WISH analysis of siblings, topbp1^cas003, topbp1^cas003; p53^+/- and topbp1^cas003; p53^-/- embryos at 4dpf, which are quantified in B. E and E′ represent partially rescued embryos; F and F′ represent well rescued embryos. (C-D′, C-D′) 23/25 siblings and 8/9 topbp1^cas003 mutants show indicated phenotype. (C′-F′) Enlarged views of CHT region indicated by arrows in the left column.

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Expression / Labeling details

Acknowledgments

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