ZFIN Image: Fortuna et al., 2015, Fig. S2

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Figure Caption

Fig. S2 Stepwise differentiation of SNs next to the DA, related to Figure 2. (AE) Whole-mount in situ hybridizations with antisense riboprobes specific for phox-2b (A, B, D) and tyrosine hydroxylase (th) (C, E) at the indicated time points. Images are ventral views (anterior is to the left) of the region between LDA-DA connection (left) and the glomerular region (G) after dissection of the yolk sac. Phox-2b positive sympathetic precursors are present around the DA at 40 hpf (A), before th+ RNA expressing cells appear at 48 hpf (C). Phox-2b and th expressions patterns expand to more anterior and posterior regions at 60 hpf (D, E). The number of embryos observed is indicated at the bottom left. Scale bar: 75 µm in A-E.

Acknowledgments

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