ZFIN Image: Fortuna et al., 2015, Fig. 2

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Figure Caption

Fig. 2

Early SN Precursors Develop Next to the DA

(A–C) Whole-mount in situ hybridizations with antisense riboprobe specific for zash-1a RNA expression at the indicated time points. Images are ventral views (anterior is to the left) of the region between the LDA-DA connection (left) and the glomerular region (G) after dissection of the yolk sac. Zash-1a⁺ sympathetic precursors are present around the DA at 40 hpf (A). At 48 hpf (B) and 60 hpf (C), zash-1a expression expands to more anterior and posterior regions. The number of embryos observed is indicated at the bottom left.

(D) Schematic representation of SN development. At 40 hpf, zash-1a+ sympathetic precursors differentiate next to the DA. At 48 hpf, they acquire HU expression and some of them become TH⁺. At 60 hpf, most of them have acquired TH expression.

DA, dorsal aorta; LDA, lateral dorsal aorta; AA, aortic arch; G, glomerulus; hpf, hours post-fertilization. Scale bar, 75 µm (A–C). See also Figure S2.

Acknowledgments

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