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Figure Caption
Fig. S2 Differential localization of dmd transcript over developmental time as detected via in situ HCR.
(a) in situ HCR of dmd-citrine (red) in ct90aGT embryos counter-stained for tpm3 (blue). tpm3 Counter-stain show the nuclei by negative contrast. Inset shows zoomed in view of active transcription sites in the nuclei. (b-d) 3D projections of confocal z-stacks of dmd transcripts stained by in situ HCR at 21hpf (b), 22hpf (c), and 25hpf (d). dmd transcript transitions from the entire somite to the somite boundaries as development progresses.
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