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Fig. 9 Gene trap insertion into the otx1b locus of tud37Gt.
(A) Schematic drawing of the otx1b locus comprising of four exons (E1-E4) encoding a homeobox domain transcription factor. White boxes represent the 5′ and 3′ untranslated regions separated by the open reading frame in pink. The mCT2aC-cassette integrated into E4. (B) Bright field images of homozygous mutant tud37Gt embryos and heterozygous siblings. In comparison to heterozygous siblings, homozygous mutant tud37Gt embryos show defects in the developing eye, fore-/midbrain, ear and heart (white arrowheads) as well as a bend body shape.
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