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Fig. S3 No defects in collagen type IV (IHC) or alignment of collagen fibers are observed at 2 dpw. A) Antibody labeling (IHC) of type IV collagen did not yield obvious differences between the different conditions. B) Blind analysis for collagen alignment (SHG) at 2 dpw indicated a nonsignificant increase in collagen mis-alignment in both control and mmp9 morphant caudal-fins. Graph represents data from experiments performed in quadruplicate and scored by an individual, single-blind analyzer. Scale bar in A represents 50 µm. Graph represents pooling with experimental numbers for no wound Control MO = 21, no wound mmp9 MO = 25, wounded Control MO = 31, wounded mmp9 MO = 32.