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Fig. 4
Acute wounding induces NFκB-dependent expression of mmp9. (A) An increased expression of mmp9 is observed by 6h post wounding (hpw) in the 2-dpf embryo. (B) A visible increase in NFκB activation is seen by 6hpw using the transgenic Tg(NFκB:gfp). The star denotes neuromast cells expressing high levels of gfp independent of wounding. The arrow indicates the edge of the wounded caudal fin and the site of increased NFκB activation. (C) Ratiometric image analysis performed in a cross of Tg(NFκB:gfp) and Tg(krt4:tdTom). (D) Early inhibition of the NFκB pathway with 30µM withaferin A resulted in a significant reduction in regeneration at 3dpw but had no effect on developmental fin length. (E) Early inhibition of the NFκB pathway resulted in an abrogation of the increased mmp9 expression in the Tg(krt4:l10a-gfp) TRAP line. *P<0.05, **P<0.01 and ****P<0.0001. Scale bars: 40µm in B,C; 100 µm in D. A represents data combined from experiments performed in triplicate; D represents data combined from experiments performed in quadruplicate and normalized to unwounded treatments.