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Fig. 7, S1 Transgenic endothelial dll4 expression is not sufficient to rescue the gpr124 mutant vascular defects.
(A) dll4 whole-mount in situ hybridization in wild-type and gpr124 mutant embryos at 36 hpf. (B) Quantification of the proportion of gpr124 morphant embryos with one or more central arteries at 60 hpf. Embryos were co-injected with gpr124 MO, tol2 transposase mRNA and pTol2-fli1:egfp, pTol2-fli1:gpr124-2A-EGFP, pTol2-fli1:EGFP-2A-gpr124, pTol2-fli1:dll4-2A-EGFP or pTol2-fli1:EGFP-2A-dll4 (C) Maximal intensity projection of a confocal z-stack of the cranial vasculature of Tg(kdrl:ras-mCherry) gpr124 morphant embryos at 60 hpf in dorsal views (anterior to the left) after transgenic endothelial overexpression (green fluorescence) of Gpr124-2A-EGFP (upper panel) or Dll4-2A-EGFP (bottom panel). Values represent means ± SD (*p < 0.05; **p < 0.01; one-way ANOVA test).