IMAGE

Fig. S6

ID
ZDB-IMAGE-150716-5
Source
Figures for Kotani et al., 2015
Image
Figure Caption

Fig. S6

Phenotypic analysis of the embryos injected with three crRNAs, tracrRNA and Cas9 protein.

(A) An uninjected embryo. (B) Tg(cmlc2:EGFP)-derived embryos injected with tracrRNA (100 pg), spns2-crRNA2 (25 pg), s1pr2-crRNA1 (25 pg) s1pr2-crRNA2 (25 pg) and Cas9 protein (400 pg). (C) Tg(cmlc2:EGFP)-derived embryos injected with spns2-gRNA1 (25 pg), s1pr2-gRNA1 (25 pg), s1pr2-gRNA2 (25 pg) and Cas9 protein (400 pg). Cardia bifida was observed in the embryo injected with multiple crRNAs, tracrRNA and Cas9 protein, whereas a normal single heart was observed in an uninjected embryo and the embryo injected with multiple gRNAs and Cas9 protein. (A-C) Ventral view with anterior at the top. White arrowheads indicate the position of the developing heart.

Acknowledgments
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