Fig. 4

Fig. 4

In adult zebrafish, BrdU labeled lateral line hair cells are lost over time under ambient conditions. (A) Schematic illustrating the experimental protocol: fish were treated with 400 µM neomycin for 1 h, then allowed to recover for 11 h, followed by a 36 h 5 mM BrdU incubation period, and finally fixed and stained at the indicated time-points. (B) Representative confocal z-stack maximum intensity projection images of neuromasts with hair cells labeled green with GFP and BrdU positive cell nuclei marked red. BrdU+ hair cells 0 days (left panel), 6 days (middle panel), and 12 days (right panel) after hair cell regeneration. Scale bar, 10 µM (C) Graph illustrating stacked averages (±s.d.) of GFP+ only hair cells (green) and GFP+ hair cells co-labeled with BrdU (red). N=2–3 adult zebrafish per condition, 30+ neuromasts per group.