ZFIN Image: Wong et al., 2015, Fig. S7

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Figure Caption

Fig. S7

Heat induction of Tg(hsp70l:dnfgfr1-EGFP) decreases vsx2 gene expression. Heat induction of the transgenic line, Tg(hsp70l:dnfgfr1-EGFP), at 48 hpf resulted in microphthalmia, with uniform gfp expression throughout the retina, which had a significantly reduced eye diameter (A–D). Standard deviation was calculated from six replicate experiments. Gene expression levels of vsx2 significantly decreased upon heat induction and gene expression levels of dmbx1a significantly increased (E). RNA from three pools of 50 embryos was quantified using RT-qPCR and standard deviation was calculated from three replicate experiments. Statistical significance was measured using Student’s t-test (p<0.05).

Acknowledgments

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Reprinted from Developmental Biology, 402(2), Wong, L., Power, N., Miles, A., Tropepe, V., Mutual antagonism of the paired-type homeobox genes, vsx2 and dmbx1, regulates retinal progenitor cell cycle exit upstream of ccnd1 expression, 216-28, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.