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Fig. 4 Canonical Wnt signalling is required persistently within a specific time window to alter cell shapes. (A-D′) β-Catenin-DAPI overlays (A-D) and β-catenin staining (A′-D′) in DMSO control (A,A′) and embryos treated with the mentioned doses of BIO drug (B-D,B′-D′). (A"-D") Comparison of nuclear β-catenin levels (A"-D") and aspect ratio plots (A′′′-D′′′) for DMSO control and embryos treated with the mentioned doses of BIO. (E-H) E-cadherin staining in embryos treated with DMSO (E) and with 0.5 mM BIO at 16 (F), 24 (G) and 30 (H) hpf. (I,J,L,M) Cell-shape analysis in DMSO control (I,L) and embryos treated with BIO from 16 hpf continuously and analysed at 30 (J) and at 36 (M) hpf. (K,N) Embryos in which the drug was (K) washed off at 24 hpf and cell shape analysis carried out at 30 hpf or (N) washed off at 30 hpf and analysed at 36 hpf. Scale bars: 10 μm. AU, arbitrary units.