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Fig. 1
Morpholino knockdown causes progressive depletion of Neurod. Whole mount immunolabeling for GFP (green) and Neurod (red) protein. Confocal images (merged z-stacks) of the dorsal pancreatic bud in Tg(neurod:EGFP) embryos at 30 hpf. (A, E, I) Control embryos; (E; inset) gray scale. Neurod UTR MO injected specimens (B, F, J) 2 ng/embryo, (C, G, K) 4 ng/embryo (D, H, L) 8 ng/embryo. Scale bar=10 µm.
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Reprinted from Developmental Biology, 402(1), Dalgin, G., Prince, V.E., Differential levels of Neurod establish zebrafish endocrine pancreas cell fates, 81-97, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.