ZFIN Image: Bührdel et al., 2015, Fig. 1

Image

Figure Caption

Fig. 1

MFM disease gene expression analysis and knock-down in zebrafish (A–E) Whole-mount antisense RNA in situ hybridization shows desmin-a and myotilin expression in skeletal muscle fibers at the 14 somite stage and 24 hpf. (C,F) At 24/48 hpf cardiac expression of desmin a/myotilin was observed (arrows). (G) As revealed by co-stainings with an antibody against Z-disk Titin (T12) or DAPI, respectively, BAG3 localizes to sarcomeric Z-disks and nuclei of zebrafish cardiomyocytes. (H) Desmin is localized at the sarcomeric Z-disk, scale bars = 10 µm. (I) Percentage of injected embryos showing a myopathic phenotype. (J) Injection of MO-Plecb blocks the splice donor site of exon 7 of Plectin b, leading to an abnormally short cDNA fragment due to skipping of exon 7. (K) Western-blot analysis shows the reduction of Desmin after injection of MO-Desma. Pan-Cadherin is used as loading control.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biochem. Biophys. Res. Commun.