ZFIN Image: Merkes et al., 2015, Fig. 3

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Fig. 3

Prehypertrophic chondrocytes of Meckel’s cartilage in MZ ewsa/ewsa fails to differentiate into hypertrophic chondrocytes.

A. Flat mounted Meckel’s cartilage of 4 dpf (left) wt/wt and (right) MZ ewsa/ewsa. B. Number of cells of Meckel’s cartilage of 4 dpf wt/wt and MZ ewsa/ewsa zebrafish (P = 0.0002). C. Lateral views (anterior to the left, dorsal to the top) of 4dpf (Left) wt/wt and (Right) MZ ewsa/ewsa visualized by in situ hybridization using probe for ihha (top panel) and colX (bottom panel). m: Meckel′s cartilage. D. Ventral views (anterior to the left) of Meckel′s cartilage of (Left) wt/wt and (Right) MZ ewsa/ewsa visualized by immunohistochemistry using (top) anti-IHH antibody (green), and anti-Collagen type II antibody (Red) (4dpf), and (bottom) anti-Collagen X antibody (green) and anti-Collagen type II antibody (Red) (5dpf).

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Acknowledgments

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