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Fig. 6 Both urp1+ and urp2+ cells are GABAergic neurons in the zebrafish embryo.
urp1 (A) and urp2 (B) expression revealed by fluorescent ISH (FITC, green) on 24 hpf-embryo, together with a fluorescent immunostaining for GAD65/67 (Alexa Fluor 546, red). Both urp1+ and urp2+ cells are GAD+ (arrows). Note that only ventral KA (KA′′) cells are doubly stained. In contrast, dorsal KA (KA′) cells are GAD+ but do not express urp1 (arrowhead). The white dash line indicates the central canal. A and B, coronal sections with dorsal up. Scale bars: 15 µm.
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