ZFIN Image: Ye et al., 2015, Fig. 4

Image

Figure Caption

Fig. 4

glucagon is required for islet development. (A) Endodermal organs isolated from 4-dpf larvae. p, pancreas; li, liver; ib, intestinal bulb. (B) qPCR of insulin (ins) and glucagon (gcga) in control and ablated larvae. ins expression was diminished and gcga expression transiently increased during regeneration (n=3 independent experiments). (C-E) gcgaMO blocks glucagon protein expression and increases the α to β cell ratio. (C-D′′) Confocal projections of 5-dpf control MO-injected (C) and gcgaMO-injected (D) Tg(gcga:GFP); Tg(ins:dsRed) larvae stained for glucagon (red). (E) Quantification of gcga:GFP⁺ α cells and ins⁺ β cells in control MO-injected (n=15) and gcgaMO-injected (n=10) islets. (F) Quantification of H2B-RFP⁺ and H2B-RFP β cells at 3 and 5dpf in control MO-injected (ne3) and gcgaMO-injected (n=5) islets. Ventral bud-derived H2B-RFP β cells are specifically diminished in gcga morphants. *P≤0.05, **P≤0.01, ***P≤0.001; ns, not significant (two-way ANOVA followed by Bonferroni post-test).

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development