ZFIN Image: Petersen et al., 2015, Fig. 4

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Figure Caption

Fig. 4

Laminin-211 Concentration Modulates In Vitro Myelination

(A–H) In vitro myelination cultures from WT or Gpr126^/ DRGs on plates coated with poly-D-lysine + 0.2 or 5 µg/ml Laminin-211. Cultures were immunostained with MBP (red) and S100 (green). The scale bar represents 100 µm.

(A and B) Three weeks after ascorbic acid (AA) addition to WT, more MBP+ immunostaining is observed on 5 µg/ml Laminin-211 than 0.2 µg/ml 3 weeks after AA addition.

(C and D) MBP+ immunostaining is not observed in Gpr126^/ SCs at any Laminin-211 concentration 3 weeks after AA addition.

(E and F) Addition of FSK leads to MBP potentiation in WT SCs on both 0.2 and 5 µg/ml Laminin-211 relative to AA alone.

(G and H) Gpr126^/ SCs have low levels of MBP in the presence of FSK and show concentration dependence for Laminin-211.

(I) Quantification of myelination cultures. Bars represent the average number of MBP-positive segments per 500 µm² ± SD. p < 0.001, Student’s t test; N.S., no significant difference.

Acknowledgments

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