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Fig. 2
Transient expression of oncogenic KRAS results in tumorigenesis in the brain region. (A) Graphic representation of driver and effector DNA constructs used for co-injection into zebrafish eggs. (B, B′) The krt5 gene promoter directed prominent transgenic expression in skin epithelial cells and other cell types, whereas the gfap promoter directed expression primarily in the CNS. (C, C′) A 9-day old krt5-transgenic fish showing focal expression of the oncogenic mCherry-KRAS in the head (outlined in white). (D, D′) A krt5-transgenic fish at 2 months of age showed gross tumor infiltration within the head. (E, E′) A gfap-transgenic fish at 2 months of age showed similar tumor infiltration in the head. (F) Both krt5- and gfap-transgenic fish developed tumors within the head region at approximately 50% penetrance by 15 months of age. Scale bars, 0.5 cm.