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Fig. 5
Specific expression of irf8 in macrophage or neutrophil lineage is sufficient to restore macrophage fates in irf8 mutants.
(A) Neutral red staining for microglia at 4 dpf in irf8 siblings and mutants in control uninjected conditions or after tol2-mediated expression of irf8 driven by tissue specific regulatory sequences. Expression of mpeg1:irf8 or lyz:irf8 was sufficient to restore some microglia in irf8 mutants (arrows), but not expression in the neurons (huc) or skin (krt4). (B) Analysis of total macrophage population by mfap4 RNA expression at 2.5 dpf after expression of irf8 in different tissues. Specific expression of mpeg1:irf8 or lyz:irf8 was also sufficient to restore macrophages on yolk sac and embryo proper (arrows) in irf8 mutants. (C) Plot showing frequency of microglia rescue. (D) Plot showing frequency of macrophage recovery. Purple denotes >25 macrophages and green shows partial recovery of less than 25 but more than 1. Numbers below bar graphs represent n, total number of embryos analyzed. sib, irf8+/+ and irf8+/-; mut, irf8-/-.