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Fig. 6 Transient ethanol exposure does not alter either hindbrain patterning or MHB formation.
(A,D,E) Control and (B,F,G) ethanol-exposed embryos display no differences in expression of mCherry in r3 and r5 at 36hpf. (C) Quantification of r3 and r5 area, showing no differences upon ethanol exposure. (D-G) In situ hybridization of pax2a in 24hpf embryos. (D-E) Control and (F-G) ethanol-exposed embryos showed no differences in the expression of pax2a in the MHB and otic placode, however in the ethanol-treated embryos pax2a was downregulated in the optic stalks. Mid-Hindbrain Boundary (MHB); optic stalk (os); otic vesicle (ov).