Fig. S2
Interrenal cell migration suppressed by 2,3-BDM was recovered following the removal of 2,3-BDM.
The interrenl tissue stained by 3β-Hsd activity assay in the control Tg(kdrl: GFP)s843 embryo continued to extend across the midline from 2.5 dpf (A-A′′) to 3 dpf (C-C′′), while migration of interrenal cells was repressed by 2,3-BDM treatment at 6 mM from 1.5 to 2.5 dpf (B-B′′) or 3 dpf (D-D′′). Migration of interrenal cells was recovered at 3 dpf as 2,3-BDM was washed out at 2.5 dpf (E-E′′). Protrusions of extending interrenal tissues (red arrows) were detected in control as well as recovered embryos. Broken white lines indicate position of the midline. Abbreviations: glomerulus (G), posterior cardinal vein (PCV). Scale bar, 50 µm.