Fig. 3
The expression of igf2b during early development of zebrafish. (A) Real-time PCR results showing the temporal expression of igf2b relative to a housekeeping gene (18s) in zebrafish embryos during the first 72 hpf; (B–N) Results of whole mount in situ hybridization showing the spatial expression of igf2b during embryogenesis. (B and C) 6 hpf (shield stage), lateral and animal view; (D and E) 12 hpf (somite stage), lateral and animal view; (F and G) 14 hpf (10-somite stage), lateral and animal view; (H and I) 18 hpf (18-somite stage), lateral and animal view; (J) 24 hpf (prim-5 stage), lateral view; (K and L) 48 hpf (long-pec stage), lateral and ventral view. (M and N) 72 hpf (protruding-mouth stage), lateral and dorsal view. FP, floor plate; MB, midbrain; OV, otic vesicle; DHB, dorsal hindbrain; HC, hyperchord; F, fin; B, brain; H, heart.
Reprinted from Gene expression patterns : GEP, 15(2), Li, J., Wu, P., Liu, Y., Wang, D., Cheng, C.H., Temporal and Spatial Expression of the four Igf Ligands and two Igf Type 1 Receptors in Zebrafish during Early Embryonic Development, 104-11, Copyright (2014) with permission from Elsevier. Full text @ Gene Expr. Patterns