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Fig. 6
Blocking hedgehog signaling restricts subsequent tooth initiation. Tooth germ dental epithelium labeled with dlx2b:GFP (A–C,G–J) and NF-KB:GFP (D–F,K–N). A–F: Tooth germs #1 and #2 in control transgenic individuals. G–I: 36–72 hpf CyA treated individuals with dlx2b:GFP expression in the position of tooth germ #1 but no evidence of tooth germ #2 (asterisk I). J: 48–72 hpf CyA treated individual with a single dlx2b:GFP+ cell in the position of tooth germ #2 (arrow). K–M: NF-κB:GFP individuals after 36–72 hpf CyA treatment with very weak and disorganized GFP expression in the tooth-forming region (arrows). N: Cluster of NF-κB:GFP expressing cells in the position of tooth germ #2 after 48–72 hpf CyA treatment (arrow). Additional stains and tooth germ orientations are as in Fig. 1. Scale bars = 10 µm in A,B.