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Fig. 4
Hedgehog inhibition starting at 36 hpf does not prevent early dental papilla formation. A–L: Tooth germ #1 after 36–48 hpf EtOH control (A–F) or CyA exposure (G–L). Arrows indicate the dental papilla. Stains are as described in Figures 1 and 2 with the addition of an Lhx6 antibody stain marking the dental papilla and adjacent nondental mesenchyme (F,L). In all cases early dental papilla formation is visible in CyA treated tooth germs, but its shape does not always appear normal. Scale bars = 10 µm in A–C.
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