Fig. 4, S2
wnt10a, fgf3 and fgf10 expression depends on Wnt/β-catenin signaling.
Control or Tg(sox10:DNerbb4) siblings were treated with DMSO, IWR-1, PD173074 or LY411575 at 32 hpf then fixed at 48 hpf for in situ hybridizations. In control siblings, inhibitor treatment has no effect on wnt10a (A–D), fgf3 (I–L) or fgf10 (Q–T) expression in interneuromast cells. wnt10a is strongly induced in Tg(sox10:DNerbb4) DMSO (E), PD173074 (G) or LY411575 (H) treated embryos but is greatly inhibited in IWR-1 treated embryos (F). Likewise, IWR-1 inhibits fgf3 (N) and fgf10 (V) expression in Tg(sox10:DNerbb4). Neither PD173074 or LY411575 effect fgf3 (O–P) or fgf10 expression (W–X) in Tg(sox10:DNerbb4).