ZFIN Image: Lush et al., 2014, Fig. 3

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Fig. 3

ErbB inhibition, after Schwann cell lateral line migration is completed, induces proliferation of interneuromast cells.

BrdU plus DMSO or AG1478 was given to Tg(SqET20:gfp) fish at 48 hpf then fixed at 14 or 24 hr post treatment. Immunohistochemistry for BrdU (red) and GFP (green) reveals no difference in BrdU incorporation within interneuromast cells between DMSO (A) or AG1478 (B) 14 hr post treatment. At 24 hr post treatment DMSO (C) treated fish show little BrdU incorporation while AG1478 (D) treated fish show increased BrdU incorporation and interneuromast cell number. Quantification of both BrdU index (E, Student’s t-test, p=0.18 for 14 hr and p=4.5E¹⁸ for 24 hr time point) and interneuromast cell number (F, Student’s t-test, p=0.69 for 14 hr and p=0.003 for 24 hr time point) shows a significant increase with AG1478 only after 24 hr.

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Acknowledgments

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